Biotechnology Assertion Reason Based Questions

The following questions consist of two statements, one labelled as Assertion (A) and the other labelled the Reason ®. Examine these two statements carefully and select the most appropriate answer codes.
A. Assertion and reason both are correct and reason is the correct explanation of Assertion.
B. Assertion and reason both are correct and reason is not the correct explanation of Assertion.
C. Assertion is correct but reason is wrong.
D. Both Assertion and Reason are false.

1. Assertion: Vector DNA and foreign DNA are cut by the same restriction endonuclease.
   Reason: Digestion of vector DNA and foreign DNA with same enzyme produces complementary sticky ends.
2. Assertion: Restriction endonuclease recognizes palindromic sequence in DNA and cuts them.
   Reason: Palindromic sequence has two unique recognition sites Pst I and Pvu I recognised by restriction endonuclease.
3. Assertion: Bacteriophage vectors are more advantages than plasmid vectors.
   Reason: Bacteriophage vector can be easily detected at the time of cloning experiments.
4. Assertion: Type I restriction endonucleases are not used in recombinant DNA technology.
   Reason: Type I restriction endonucleases recognise specific sites within the DNA but do not cut these sites.
5. Assertion: Amplification of a gene of interest can be done by polymerous chain reaction.
   Reason: It is possible to amplify DNA segment approximately 1 billion times within a span of one day.
6. Assertion: Golden Rice is a transgenic rice have gene for beta- carotene.
   Reason: The consumption of golden rice by men can make up the deficiency of vitamin A preventing blindness.
7. Assertion: ADA is an enzyme which is deficient in genetic disorder SCID
   Reason: SCID disorder cannot be treated by ADA gene therapy.
8. Assertion: Cholesterol is one of the most important steroid in animal and human beings.
   Reason: Cholesterol is present in cell wall of animals and help in cell wall extension.
9. Assertion: Molecular probes are available for diagnosing genetic disorders, example., Duchenne muscular dystrophy, Cystic fibrosis, Tay- sach’s disease.
   Reason: The molecular probes are usually double stranded pieces of DNAs, labelled with radio isotopes such as 32p.
10. Assertion: In gene therapy, new gene is introduced only into the somatic cells.
    Reason: Genetic modification is done to replace faulty genes by normal functional gene.
11. Assertion: Mouse is the most preferred mammal for studies on gene transfer.
    Reason: Mouse possesses features like short oestrous cycle and gestation period, relatively short generation time, production of several offsprings per pregnancy, etc.
12. Assertion: Transgenic food may cause toxicity or produce allergy.
    Reason: Transgenic plants have high nutrient content.
13. Assertion: Cotton bollworms are killed by ingesting the leaves of the transgenic plant.
    Reason: Bt toxin binds with the midgut wall of the insect and make pores in them.
14. Assertion: In recombinant DNA technology human gens are often transferred into bacteria or yeast.
    Reason: Both bacteria and yeast multiply very fast to form huge population which express the desired gene.
15. Assertion: The kneaded flour shows leavening when yeast is added to it.
    Reason: Enzymes secreted by yeast do not cause leavening.
16. Assertion: All genetically modified organisms are rich in chimeric DNA (rDNA).
    Reason: Chimeric DNA constitutes genes of two similar organisms.
17. Assertion: Genetically modified microbes help in crop protection.
    Reason: Transgenic bacteria control insects by producing endotoxins.
18. Assertion: Organs of pig such as heart, pancreas, etc., for human use can be grown through transgenic animals.
    Reason: Transgenic pigs show improved growth and meat production.
19. Assertion: PCR is routinely used for early diagnosis of HIV in suspected AIDS patients.
    Reason: PCR can detect low amounts of DNA.
20. Assertion: Bio patents are awarded for biological entities and all product derived from them.
    Reason: Patent on use of turmeric for wound healing was canceled in 2008.

Answer Sheet

1. A
2. C
3. A
4. A
5. B
6. A
7. C
8. C
9. C
10. B
11. A
12. B
13. A
14. A
15. C
16. C
17. B
18. B
19. B
20. C